Electrophoresis Principle and its types
Electrophoresis Electrophoresis is a technique used to separate macromolecules in a fluid or gel based on their charge, binding affinity, and size under an electric field. In the year 1807, Ferdinand Frederic Reuss was the first person to observe electrophoresis. He was from Moscow State University. Anaphoresis is the electrophoresis of negative charge particles or anions whereas cataphoresis is electrophoresis of positive charge ions or cations. Electrophoresis has a wide application in separating and analysing biomolecules such as proteins, plasmids, RNA, DNA, nucleic acids. Electrophoresis Principle and its types: Charged macromolecules are placed in the electric field move towards the negative or positive pole based on their charge. Nucleic acid has a negative charge and therefore it migrates towards the anode. This technique is divided into two types viz slab electrophoresis and capillary electrophoresis. Types of Electrophoresis: 1. ...